G4 biology

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<p><i>G4 Biology, Volume 695 </i>in the <i>Methods in Enzymology</i> series, highlights high value topics such as Assays to measure G4 resolution by DNA helicases, In gel staining methods of G4 DNA and RNA structures, Methods to probe the folding pathways of four-stranded intercalated cytosine-rich motifs at single base-pair resolution, Analyzing the effect of transcription coupled R-loop formation on G4 formation, Integrated Structural Biology for the study of higher-order G4 structures, Advancing the Study of Protein-G4 Interactions in DNA Repair: Insights from Biolayer Interferometry and Circular Dichroism Spectroscopy, Immunofluorescence microscopy (IF) of G-quadruplex and R-loops, and more. Additional chapters delve into Production of the anti-G-quadruplex antibody BG4 for efficient ChIP-seq and CUT&Tag analysis: from plasmid quality control to validation, Utility of intercalator displacement assays for screening of ligands for i-motif DNA structures, Potentiometric titrations to